Answer: Some pathogens and eggs of pear trees parasitize on dead leaves and overwintering. They need to clean up the debris in the pear orchard and burn them in a concentrated manner. This can eliminate overwintering pathogens and eggs. At the same time, change the soil and fertilize the old soil at the base of the pear tree to see the roots and cut off the roots. Sprinkle the roots and rot, and then replace them with new soil to prevent root rot. Then, under the canopy, ring-shaped, radial, and strip-shaped fertilization methods were applied to farmyard manure, phosphate fertilizer, and potash fertilizer, and appropriate nitrogen fertilizers were applied. In the end, the park was turned over to use the natural conditions of low temperature and drought in winter to turn the overwintered pests in the soil and freeze them to death or be eaten by birds. The depth of the garden should be 20-25 cm, and it is good to advance in soil freezing. Gardening can not only eliminate overwintering pests, but also improve soil physical and chemical properties, improve soil structure, and improve the ability of pear orchard soils to retain water and maintain water in winter.
The RNA purification kit is used to purify and recover RNA molecules transcribed in vitro and total RNA extracted from various materials, which can effectively remove contaminating impurities in RNA samples. The recovery rate of this product can reach 80%, and the OD260/OD280 ratio of the obtained RNA is generally about 2.0, which can be directly used in subsequent sensitive experiments (such as microarray analysis, fluorescence RT-PCR, etc.). With the deepening of transcriptomics, the complexity of RNA types, expression regulation and functions is far beyond our imagination.
Removing ribosomal RNAs that account for more than 80% helps to focus sequencing on less abundant but informative RNAs. At present, the removal of rRNA is mainly through the combined use of probe and RNase H. The processed RNA will be mixed with many digestion products, enzymes and ions, which is not conducive to the subsequent construction of RNA library. Take the Columnar RNA Purification Kit as an example. Trizol is a ready-to-use reagent that can be used to purify total RNA from tissues and cells. This is a single-phase solution of phenol and guanidine isothiocyanate that facilitates lysis of tissues and cells, inhibiting RNases to maintain RNA integrity.
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